Essentials of Practical Biochemistryis a meticulously crafted learner-centered educational resource designed to meet the evolving standards of medical education as per the 2024 revised CBME guidelines mandated by the National Medical Commission (NMC). This book takes a dual approach, combining deep, meaningful learning with structured, competency-aligned assessments emphasizing that “assessment drives student learning.” By integrating core biochemical concepts with practical, clinically oriented assessment methodologies, it offers a comprehensive guide that not only facilitates knowledge retention but also promotes the application of theoretical concepts to real-world clinical scenarios.